rabbit anti leptin receptor antibody  (Bioss)

Journal: Frontiers in Immunology

Article Title: Leptin deficiency in CD8 + T cells ameliorates non-segmental vitiligo by reducing interferon-γ and Granzyme B

doi: 10.3389/fimmu.2023.1158883

Figure Lengend Snippet: LEPR expression in CD8 + T cells from vitiligo patients was increased. (A, B) Double immunofluorescence staining results of LEPR and CD8 in vitiligo lesions and normal skin (Vitiligo: N = 5, NC: N = 5). (A) CD8 was represented by green fluorescence and LEPR was represented by red fluorescence. (B) The expression of LEPR in CD8 + T cells in Vitiligo lesions was significantly higher. (C) The serum levels of leptin were evaluated by ELISA (Vitiligo: N=53, HC: N=36). (D, E) Representative flow cytometric profiles and data plots of CD8 + T cells in PBMCs are shown (Vitiligo: N=5, HC: N=5). * P < 0.05, ** P < 0.01.

Article Snippet: The following antibodies were used: Alexa Flour 647 Donkey anti-rabbit IgG (BioLegend), Rabbit Anti-Leptin receptor antibody (bs-0410R, Bioss), APC-Cy7 anti-human CD4 (BD Biosciences), Percp-Cy5.5 anti-human CD8 (BD Biosciences), PE anti-human Perforin (BD Biosciences), FITC anti-human/mouse Granzyme B (Granzyme B, BioLegend), PE-Cy7 anti-human IFN-γ (BioLegend), Zombie Aqua Fixable Viability Kit (BioLegend), APC-Cy7 anti-mouse CD4 (Abcam), Percp-Cy5.5 anti-mouse CD8 (Abcam), PE anti-mouse Perforin (Abcam), FITC anti-mouse/human granzyme B (BioLegend), PE-Cy7 anti-mouse IFN-γ (Abcam).

Techniques: Expressing, Double Immunofluorescence Staining, Fluorescence, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Immunology

Article Title: Leptin deficiency in CD8 + T cells ameliorates non-segmental vitiligo by reducing interferon-γ and Granzyme B

doi: 10.3389/fimmu.2023.1158883

Figure Lengend Snippet: Leptin enhances the expression of cytotoxic cytokines from CD8 + T cells in vitro . (A, B) Gating strategy, representative flow cytometric plots, and statistical analysis of the percentage of cell subsets were shown. (C) After 72 hours of leptin stimulation, the protein levels of IFN-γ, perforin, and Granzyme B from normal PBMCs were detected by ELISA. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The following antibodies were used: Alexa Flour 647 Donkey anti-rabbit IgG (BioLegend), Rabbit Anti-Leptin receptor antibody (bs-0410R, Bioss), APC-Cy7 anti-human CD4 (BD Biosciences), Percp-Cy5.5 anti-human CD8 (BD Biosciences), PE anti-human Perforin (BD Biosciences), FITC anti-human/mouse Granzyme B (Granzyme B, BioLegend), PE-Cy7 anti-human IFN-γ (BioLegend), Zombie Aqua Fixable Viability Kit (BioLegend), APC-Cy7 anti-mouse CD4 (Abcam), Percp-Cy5.5 anti-mouse CD8 (Abcam), PE anti-mouse Perforin (Abcam), FITC anti-mouse/human granzyme B (BioLegend), PE-Cy7 anti-mouse IFN-γ (Abcam).

Techniques: Expressing, In Vitro, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Immunology

Article Title: Leptin deficiency in CD8 + T cells ameliorates non-segmental vitiligo by reducing interferon-γ and Granzyme B

doi: 10.3389/fimmu.2023.1158883

Figure Lengend Snippet: Leptin deficiency in CD8 + T cells ameliorated vitiligo development in mice. (A) The hair decolorization areas of mice with vitiligo induced by monobenzone are shown, marked with red circles. (B) HE staining showed that the inflammatory cells of the Lep KO mice were significantly reduced compared with the WT group. (C) The heatmap shows the differential expression of vitiligo-related genes between WT and Lep KO mice after monobenzone was administrated. (D) RT-qPCR was used to verify the differentially expressed genes in CD8 + T cells from spleens of vitiligo mice induced by monobenzone in the Lep KO group and WT group. * P < 0.05, *** P < 0.001.

Article Snippet: The following antibodies were used: Alexa Flour 647 Donkey anti-rabbit IgG (BioLegend), Rabbit Anti-Leptin receptor antibody (bs-0410R, Bioss), APC-Cy7 anti-human CD4 (BD Biosciences), Percp-Cy5.5 anti-human CD8 (BD Biosciences), PE anti-human Perforin (BD Biosciences), FITC anti-human/mouse Granzyme B (Granzyme B, BioLegend), PE-Cy7 anti-human IFN-γ (BioLegend), Zombie Aqua Fixable Viability Kit (BioLegend), APC-Cy7 anti-mouse CD4 (Abcam), Percp-Cy5.5 anti-mouse CD8 (Abcam), PE anti-mouse Perforin (Abcam), FITC anti-mouse/human granzyme B (BioLegend), PE-Cy7 anti-mouse IFN-γ (Abcam).

Techniques: Staining, Expressing, Quantitative RT-PCR

Journal: Nature Metabolism

Article Title: A subset of dopamine receptor-expressing neurons in the nucleus accumbens controls feeding and energy homeostasis

doi: 10.1038/s42255-024-01100-0

Figure Lengend Snippet: a , Diagram indicating the MCH + , orexin-A + , GABA + and LepR + neurons in the LH. b , c , Example images of non-connected MCH + ( b ) and orexin-A + neurons (gray) ( c ) and Serpinb2 + neuron terminals (green fibers with red puncta) in the LH. Scale bars, 50 μm (left), 20 μm (right). Arrows indicate somas. d , e , Images showing the colocalization of Serpinb2 + neuron terminals (green fibers with red puncta) with GABA + ( d ) and LepR + (gray) ( e ) as indicated by arrows. Scale bars, 50 μm (left), 20 μm (right). One technical replicate of three biological replicates. f , Quantification of Serpinb2 + neuron terminal connected cells. Percentage represents eYFP + MCH + /eYFP + DAPI + , eYFP + orexin-A + /eYFP + DAPI + , eYFP + GABA + /eYFP + DAPI + or eYFP + LepR + /eYFP + DAPI + . n = 3 sections from three mice. One-way ANOVA test, F 3,8 = 101. g , The timeline of monosynaptic retrograde rabies tracing of LH LepR neurons. n = 4 biological replicates. h , Representative images showing the location of starter LH LepR neurons in a LepR-Cre mouse; mCherry-TVA (red), rabies-GFP (green) and DAPI (blue). Scale bars, 100 µm (left), 50 µm (right). i , Representative histological images with cells retrogradely labeled from LH LepR neurons (green) and Serpinb2 + neurons (red). Scale bars, 200 µm (left), 50 µm (right). j , cFos detection in the LH of DREAAD-treated Serpinb2 -Cre mice. Virus expression in NAcSh (top); mCherry (red), cFos (green) and DAPI (blue). CNO-induced cFos immunofluorescence signals were observed in the LH; bottom panels are enlarged images of the squares above. Scale bars, 100 µm (top), 200 µm (middle) and 50 µm (bottom). k , The number of cFos + cells ( n = 4 sections from four mice). One-way ANOVA test, F 2,9 = 69.51. l , Diagram showing cannula implantation in LH for leptin delivery (left, middle). Results of total food consumption in 3 h by fasted mice with different doses of leptin administration (right). Scale bar, 500 μm. One-way ANOVA test, F 3,10 = 6.025. m , Same as k except food consumption is quantified under different conditions with or without Serpinb2 + neuron activation in the presence or absence of 1 μg (middle) or 300 ng (right) of leptin delivery. One-way ANOVA test. F 3,24 = 14.72 (left), F 3,24 = 5.285 (right). Data are presented as mean ± s.e.m. The graphics of the mouse and syringe in l and m were created with BioRender.com .

Article Snippet: For immunofluorescence, cryostat sections were collected and incubated overnight with blocking solution (1× PBS containing 5% goat serum, 5% BSA and 0.1% Triton X-100) and then incubated with the following primary antibodies, diluted with blocking solution, for 1 day at 4 °C: rabbit anti-cFos (1:2,000; Synaptic systems, cat. no. 226003), chicken anti-GFP (1:2,000; Aves Labs, cat. no. GFP-1010), chicken anti-mCherry (1:2,000; Novus Biologicals, cat. no. NBP2-25158), mouse anti-orexin-A (KK09) (1:500; Santa Cruz Biotechnology, cat. no. sc-80263), rabbit anti-GABA (1:1,000; Sigma, cat. no. A2052), rabbit anti-MCH (1:20,000; Phoenix Pharmaceuticals, cat. no. H-070-47) and rabbit anti-leptin receptor (1:1,000; Abcam, cat. no. 104403).

Techniques: Labeling, Virus, Expressing, Immunofluorescence, Activation Assay

Journal: Cell Metabolism

Article Title: Adipo-glial signaling mediates metabolic adaptation in peripheral nerve regeneration

doi: 10.1016/j.cmet.2023.10.017

Figure Lengend Snippet:

Article Snippet: Rabbit Anti-Leptin/Obese Receptor (OBRb) , Bio Trend , Cat#. OBR12-A; RRID: AB_1611942.

Techniques: Recombinant, Electron Microscopy, Protease Inhibitor, Picogreen Assay, Microarray, RNA Sequencing Assay, Real-time Polymerase Chain Reaction, Software